Structure
Polysucrose is a polymer synthesised by cross-linking sucrose with epichlorohydrin. FITC-CM-polysucroses are manufactured by reacting selected polysucrose fractions with an activated carboxymethyl derivative in alkali whereby O-carboxymethyl groups are introduced along the polysucrose chain. The carboxyl content is approximately 5% which is equivalent to about one CM group for every five glucose units. Thereafter, fluorescein (FITC) groups are introduced by reaction with fluorescein isothiocyanate. Polysucrose has a more spherical structure and is less flexible compared to dextran.
Spectral data
FITC-CM-polysucrose has an excitation maximum of 490 – 495 nm and an emission maximum of 520 ± 5 nm at pH 9.
Storage and stability
FITC-CM-polysucrose is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature.
Solubility
FITC-CM-polysucrose dissolves readily in water.
Application
FITC-CM-polysucroses are mostly used for studies of permeability and microcirculation. The carboxyl groups will impart an overall negative charge to the molecule, which may be valuable in gaining information on the permeability characteristics of cell membranes and tissues. FITC-CM-polysucrose has played an interesting role in elucidating the properties of the glomerular membrane. Read more about applications here.
