Cryopreservation is a process employed for the preservation of biological matter such as cells, tissues and organs. The preservation is performed at low temperatures, typically at liquid nitrogen temperature at −196 °C. The application scope of cryopreservation is very large spanning from preservation of cells or organs to cryosurgery, blood transfusion and in vitro fertilization (1). The process requires the use of suitable media involving compounds or derivatives, also called cryoprotectants. These needs to be of low toxicity, have a strong anti-freezing capacity and a good post-thawing performance.




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  1. Han, Y.-J. et al. Stem Cells from Cryopreserved Human Dental Pulp Tissues Sequentially Differentiate into Definitive Endoderm and Hepatocyte-Like Cells in vitro. Int. J. Med. Sci. 14, 1418–1429 (2017).






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