FITC (Fluorescein isothiocyanate) is a derivative of fluorescein. FITC exhibits an excitation maximum at λ = 495 nm and emission maximum at approximately λ = 519 nm. FITC can be easily used as labelling agent of various substrates such as proteins or polysaccharides. Isothiocyanate easily reacts with nucleophiles such as amines under mild conditions. FITC can also be used in flow cytometry.
Cibacron Blue 3G-A is an anionic anthraquinone dye. It is as a ligand in affinity chromatography for the purification of proteins, enzymes and biopolymers. Cibacron Blue 3G-A has also been employed in the textile industry for many years. It has shown tremendous protein binding capacity and can readily be immobilized on polymers containing a hydroxyl group.