TRITC (5- and 6-isothiocyanate isomers) is a prominent member of the family of rhodamine dyes. It exhibits an excitation maximum at λ = 560 nm and emission maximum at approximately λ = 590 nm with bright orange fluorescence (values determined in DMSO solutions). TRITC is an indispensable fluorescent labeling agent and has a wide number of applications, such as in cell imaging, drug delivery, and studies involving permeability and microcirculation.