Below you will find the answers to the most frequently asked questions.
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Dextran Sulfate Sodium (DSS) for colitis
Dosage
The dose range lies between 2-3.5% DSS. If you have not run any studies before, we recommend testing a few animals at 2%, 2.5% and 3% DSS.
Experimental parameters
There are three important parameters when performing experiments with DSS:
Dosage The dose range lies between 2-3.5% DSS. If you have not run any studies before, we recommend testing a few animals at 2%, 2.5% and 3% DSS.
The animals being studied. You must obtain a certificate from the supplier defining the following:
Age-group
Sexes
Strain
Conditions during treatment
Chow
Amount of DSS-treated water that the animals drink
Exercise levels
Light levels
Temperatures
Other room conditions
Precipitations
Dissolving DSS in hard water can cause cloudy solutions or precipitations. Use soft water or ultra-pure water if the problem appears.
Solubility
High molecular weight dextran
All high molecular dextran derivatives (≥150 kDa), with or without an attached dye, will be soluble when heated to 50 °C during continuous stirring for 30 min.
FITC Inulin and blue dextran
FITC inulin, and in some cases also blue dextrans, will be soluble when heated to 80 °C during continuous stirring.
DEAE- and CM-dextran
DEAE- and CM-dextrans will be dissolved faster if you add the powder to water (not the other way around) during continuous stirring.
Dextran with a high polar aromatic substance
Dextran derivatives with a molecular weight ≥1000 kDa with a high polar aromatic substance are recommended to be heated to 50 °C during continuous stirring. Even if the substance looks dissolved; it can form an invisible globular structure in solution.
Other
Shipping conditions
The shipping condition for all our products is room temperature.
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