FAQ

FAQ

Below you will find the answers to the most frequently asked questions.

If you don’t find the answer please send an e-mail to info@tdblabs.se or give us a call at +46 73 0608200. Thank you!

Dextran Sulfate Sodium (DSS) for colitis

Dosage

The dose range lies between 2-3.5% DSS. If you have not run any studies before, we recommend testing a few animals at 2%, 2.5% and 3% DSS.

Experimental parameters

There are three important parameters when performing experiments with DSS:

  1. Dosage The dose range lies between 2-3.5% DSS. If you have not run any studies before, we recommend testing a few animals at 2%, 2.5% and 3% DSS.
  2. The animals being studied. You must obtain a certificate from the supplier defining the following:
    • Age-group
    • Sexes
    • Strain
  3. Conditions during treatment
    • Chow
    • Amount of DSS-treated water that the animals drink
    • Exercise levels
    • Light levels
    • Temperatures
    • Other room conditions

Precipitations

Dissolving DSS in hard water can cause cloudy solutions or precipitations. Use soft water or ultra-pure water if the problem appears.

Solubility

High molecular weight dextran

All high molecular dextran derivatives (≥150 kDa), with or without an attached dye, will be soluble when heated to 50 °C during continuous stirring for 30 min.

FITC Inulin and blue dextran

FITC inulin, and in some cases also blue dextrans, will be soluble when heated to 80 °C during continuous stirring.

DEAE- and CM-dextran

DEAE- and CM-dextrans will be dissolved faster if you add the powder to water (not the other way around) during continuous stirring.

Dextran with a high polar aromatic substance

Dextran derivatives with a molecular weight ≥1000 kDa with a high polar aromatic substance are recommended to be heated to 50 °C during continuous stirring. Even if the substance looks dissolved; it can form an invisible globular structure in solution.

Other

Shipping conditions

The shipping condition for all our products is room temperature.

 

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