Eosin-Y-dextran 10

CAS Number: N/A
Alternative Name: EYD10
Eosin-Y-dextran 10 (EYD10) is a dextran derivative that is labelled with the Eosin Y dye and it is currently produced at a molecular weight of 10kDa.

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Structure and physical properties
Eosin-Y-dextran 10 (EYD10) is a dextran-based derivative labeled with the dye Eosin Y (2-(2,4,5,7-tetrabromo-6-oxido-3-oxo-3H-xanthen-9-yl) benzoate; CAS No: 17372-87-1). Dextran is marked using a special technique created by TdB Labs, which enables a significant level of functionalization (Fig. 1). The method utilizes the amino groups of a previously functionalized dextran to create a linker that involves amide groups. This linker provides considerable spacing between the substrate and the fluorophore, while also offering H-bonding opportunities (intramolecular and/or intermolecular, e.g., with solvent molecules).

Owing to the presence of Eosin Y, EYD10 exhibits strong orange fluorescence (excitation max. at λ=532 nm and fluorescence max. at λ=555 nm in water; see Fig. 2). The fluorescence quantum yield for EYD10 sample with a degree of fluorescent labelling of 0.003 mmol EY/g sample has been determined to be Φ=0.38.

EYD10 is sensitive to pH due to the presence of Eosin Y, a tetrabrominated derivative of fluorescein that exhibits a similar dependency on pH like its parent compound. A gradual decrease in the absorbance band of EYD10 is observed when lowering the pH from 9 to 1 (Fig. 3). Similarly, the fluorescence of EYD10 drops by a factor of approximately 7 upon the decrease of pH from 9 to 1.

Storage and stability
EYD10 should be stored in a well-sealed container in the dark, at room temperature, and has a shelf-life of 6 years under these conditions.

Solubility
EYD10 is delivered as a dark ruby red color amorphous powder readily soluble at concentrations close to 100 mg/ml in water and 50 mg/ml in DMSO, where mild heating (up to max 60^oC) might be required. However, it is insoluble in methanol and ethanol.

Application
EYD10 is suitable for a range of potential applications such as in cell permeability studies, as a fluorescent nanocarrier for drug delivery applications and research thereof, and as a pH indicator.

References

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