Our TRITC-derivatives are produced by conjugating tetramethyl-rhodamine B isothiocyanate (TRITC) to polysaccharide fractions by a stable thiocarbamate linkage. A mixture of 5- and 6-amino-tetramethyl-rhodamine B is converted to the isothiocyanate (TRITC). Our TRITC products are supplied as a pink powder.
Spectral data
The excitation maximum for TRITC is 550 nm and the emission maximum is 572 nm. The fluorescence is less dependent on pH than for fluorescein. A slight increase is noted around pH 6-7.
Derivatives
We offer the following products; TRITC-dextran, TRITC-lysine-dextran, TRITC-polysucrose and TRITC-hyaluronic acid.
Applications
The main application for TRITC-dextran is permeability and microcirculation. Lysine-derivatives are often used for fixation and conjugation in order to preserve components in a “life-like” state. Read more about application here.