Eosin-Y-dextran 10

Eosin-Y-dextran 10

CAS Number: N/A
Alternative Name: EYD10
Eosin-Y-dextran 10 (EYD10) is a dextran derivative that is labelled with the Eosin Y dye and it is currently produced at a molecular weight of 10kDa.

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Structure and physical properties
Eosin-Y-dextran 10 (EYD10) is a dextran-based derivative labeled with the dye Eosin Y (2-(2,4,5,7-tetrabromo-6-oxido-3-oxo-3H-xanthen-9-yl) benzoate; CAS No: 17372-87-1). Dextran is marked using a special technique created by TdB Labs, which enables a significant level of functionalization (Fig. 1). The method utilizes the amino groups of a previously functionalized dextran to create a linker that involves amide groups. This linker provides considerable spacing between the substrate and the fluorophore, while also offering H-bonding opportunities (intramolecular and/or intermolecular, e.g., with solvent molecules).


EYD structure

Fig. 1 Structure of Eosin-Y-dextran 10 kDa (ratio x/y depends on the load of fluorescent labelling; R bulky substituent not displayed).

Owing to the presence of Eosin Y, EYD10 exhibits strong orange fluorescence (excitation max. at λ=532 nm and fluorescence max. at λ=555 nm in water; see Fig. 2). The fluorescence quantum yield for EYD10 sample with a degree of fluorescent labelling of 0.003 mmol EY/g sample has been determined to be Φ=0.38.


EYD absorbance and fluorescence spectra

Fig. 2 Absorbance and fluorescence spectra of EYD10 recorded in borate buffer (pH=7.0).  

EYD10 is sensitive to pH due to the presence of Eosin Y, a tetrabrominated derivative of fluorescein that exhibits a similar dependency on pH like its parent compound. A gradual decrease in the absorbance band of EYD10 is observed when lowering the pH from 9 to 1 (Fig. 3). Similarly, the fluorescence of EYD10 drops by a factor of approximately 7 upon the decrease of pH from 9 to 1.


EYD pH dependence

Fig. 3 Spectrophotometry experiments indicating the dependence on pH of absorbance (left) and emission (right) spectra of EYD10 recorded in water at 25oC. Arrows indicate the observed decrease of absorbance or emission intensity upon pH decrease from 9 to 1.

Storage and stability
EYD10 should be stored in a well-sealed container in the dark, at room temperature, and has a shelf-life of 6 years under these conditions.

EYD10 is delivered as a dark ruby red color amorphous powder readily soluble at concentrations close to 100 mg/ml in water and 50 mg/ml in DMSO, where mild heating (up to max 60oC) might be required. However, it is insoluble in methanol and ethanol.

EYD10 is suitable for a range of potential applications such as in cell permeability studies, as a fluorescent nanocarrier for drug delivery applications and research thereof, and as a pH indicator.


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