Our TRITC-derivatives are produced by conjugating tetramethyl-rhodamine B isothiocyanate (TRITC) to polysaccharide fractions by a stable thiocarbamate linkage. A mixture of 5- and 6-amino-tetramethyl-rhodamine B is converted to the isothiocyanate (TRITC). Our TRITC products are supplied as a pink powder.
The excitation maximum for TRITC is 550 nm and the emission maximum is 572 nm. The fluorescence is less dependent on pH than for fluorescein. A slight increase is noted around pH 6-7.
We offer the following products; TRITC-dextran, TRITC-lysine-dextran, TRITC-polysucrose and TRITC-hyaluronic acid.
The main application for TRITC-dextran is permeability and microcirculation. Lysine-derivatives are often used for fixation and conjugation in order to preserve components in a “life-like” state. Read more about application here.
Tetramethylrhodamine Hyaloronic Acid
Hyaluronic acid (HA) can be obtained from various sources, for example rooster combs or Streptococcus equi. We can supply derivatives of either of these qualities labelled with or with tetramethylrhodamine (T-Ha). The molecular weightis approximately 6 MDa.
TRITC-lysine-dextran is a dextran derivative carrying both free primary amines and free carboxylates as well as the fluorescent dye Tetramethylrhodamine (TRITC). TdB Labs produce TRITC-lysine-dextran from 4 kDa to 500 kDa.