FITC-CM-polysucrose

FITC-CM-polysucrose

FITC-CM-polysucroses are standard FITC-polysucroses carrying carboxymethyl-substituents. Polysucrose (renamed from Ficoll™) is a polymer which displays excellent biocompatibility. The CM (carboxymethyl) group gives the product anionic (negative) charge. All batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. TdB Labs produces FITC-CM-polysucroses with a molecular weight of 70 kDa and 400 kDa and it is supplied as a yellow powder.

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Structure
Polysucrose is a polymer synthesised by cross-linking sucrose with epichlorohydrin. FITC-CM-polysucroses are manufactured by reacting selected polysucrose fractions with an activated carboxymethyl derivative in alkali whereby O-carboxymethyl groups are introduced along the polysucrose chain. The carboxyl content is approximately 5% which is equivalent to about one CM group for every five glucose units. Thereafter, fluorescein (FITC) groups are introduced by reaction with fluorescein isothiocyanate. Polysucrose has a more spherical structure and is less flexible compared to dextran.

Spectral data
FITC-CM-polysucrose has an excitation maximum of 490 – 495 nm and an emission maximum of 520 ± 5 nm at pH 9.

Storage and stability
FITC-CM-polysucrose is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature.

Solubility
FITC-CM-polysucrose dissolves readily in water.

Application
FITC-CM-polysucroses are mostly used for studies of permeability and microcirculation. The carboxyl groups will impart an overall negative charge to the molecule, which may be valuable in gaining information on the permeability characteristics of cell membranes and tissues. FITC-CM-polysucrose has played an interesting role in elucidating the properties of the glomerular membrane. Read more about applications here.

References

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  1. Srikantha, N. et al. Influence of molecular shape, conformability, net surface charge, and tissue interaction on transscleral macromolecular diffusion. Exp. Eye Res. 102, 85–92 (2012).
  2. Goldberg, S., Harvey, S. J., Cunningham, J., Tryggvason, K. & Miner, J. H. Glomerular filtration is normal in the absence of both agrin and perlecan–heparan sulfate from the glomerular basement membrane. Nephrol Dial Transplant 24, 2044–2051 (2009).
  3. Harvey, S. J. et al. Disruption of Glomerular Basement Membrane Charge through Podocyte-Specific Mutation of Agrin Does Not Alter Glomerular Permselectivity. The American Journal of Pathology 171, 139–152 (2007).
  4. Greive, K. A. et al. Glomerular Permselectivity Factors Are Not Responsible for the Increase in Fractional Clearance of Albumin in Rat Glomerulonephritis. The American Journal of Pathology 159, 1159–1170 (2001).

Technical documents

How to order

Visit our webshop to see the molecular weights and pack sizes available. Please send an e-mail to order@tdblabs.se if you would like to receive a quote, place a bulk order or if you wish to place your order manually.

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