pH probes

pH probes

Spectral data
The fluorophores FITC/TRITC/AR have an excitation maximum of 493/550/585 nm and an emission maximum of 517/575/600 nm.

Derivatives
We offer the following products; FITC-TRITC-dextran 500 and FITC-AR-dextran 20.

Applications
The fluorescence (as well as the absorbance) of FITC is highly dependent on pH while that of TRITC and AR remains nearly unchanged between pH 2 and 10. This makes FTD500 and FARD20 highly suitable for applications that allow accurate determination and monitoring of pH in living cells or tissues at a wide range of pH spanning from 3.5 to 8.0. Dual fluorescent labeled dextrans thus serve as invaluable products for intravital microscopy.

FTD500 powder

FITC-TRITC- dextran 500

FITC-TRITC-dextran (FTD) is a dextran derivative that is labelled with two fluorophores, FITC and TRITC and it is currently produced at a molecular weight of 500kDa.

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FARD20 powder

FITC-Antonia Red- dextran 20

FITC-Antonia Red-dextran (FARD) is a dextran derivative that is labelled with two fluorophores, FITC and Antonia Red and it is currently produced at a molecular weight of 20kDa.

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