FITC-TRITC-dextran 500

FITC-TRITC-dextran 500

CAS Number: N/A
Alternative Name: FTD

FITC-TRIC-dextran (FTD) is a dextran derivative that is labelled with two fluorophores, FITC and TRITC (see Fig 1) and it is currently produced at a molecular weight of 500 kDa.

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Structure and physical properties
Structurally, each of the fluorophores (FITC and TRITC) are bound to dextran through thiourethane linkages (Fig 1). The degree of substitution (DS) lies in the range of 0.001 to 0.020 mol of dye per mole of glucose units for each of the fluorophores. The molar ratio of FITC/TRITC bound to dextran can vary between 1:1 and 2:1 and it is assessed spectrophotometrically at a neutral pH.

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Fig 1. Structural representation of FITC-TRITC-dextran (FTD)

As a result of the dual fluorescent labelling of dextran, FITC-TRITC-dextran exhibits fluorescence emission at λfl=517±5nm when photoexcited at λex=493 nm (due to FITC) and at λfl=575±5nm when photoexcited at λex=550 nm (due to TRITC).

What renders this TdB Labs product unique is that the fluorescence (as well as the absorbance) of one of the fluorophores (FITC) is highly dependent on pH while the corresponding ones of the other fluorophore (TRITC) remains nearly unchanged between pH 2 and 10 (see Fig 2). This allows for accurate determination of pH in living cells or tissues and thereby renders this product an invaluable tool for intravital microscopy.

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Fig 2. UV-Vis spectra depicting the dependence of two visible features of FTD upon varying pH

Storage and stability
FTD500 should be stored in a well-sealed container in the dark, at room temperature.

FTD500 is delivered as a pink powder, that is highly soluble in water and DMSO. However, it is incompatible with strong oxidizing agents.

FTD500 is suitable for applications requiring accurate detection and monitoring of pH in living cells or tissues, where it can operate at a wide range of pH spanning from 3.5 to 8.0.


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How to order

Visit our webshop to see the molecular weights and pack sizes available. Please send an e-mail to if you would like to receive a quote, place a bulk order or if you wish to place your order manually.

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