CAS Number: N/A Alternative Name: FARD
FITC-Antonia Red-dextran (FARD) is a dextran derivative that is labelled with two fluorophores, FITC and Antonia Red (see Fig 1) and it is currently produced at a molecular weight of 20 kDa.
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Structure and physical properties
Structurally, each of the fluorophores (FITC and Antonia Red) are bound to dextran through thiourethane linkages (Fig 1). The degree of substitution (DS) lies in the range of 0.001 to 0.020 mol of dye per mole of glucose units for each of the fluorophores. The molar ratio of FITC/AR bound to dextran can vary between 1:1 and 1:3 and it is assessed spectrophotometrically at a neutral pH.
As a result of the dual fluorescent labelling of dextran, FITC-Antonia Red-dextran exhibits fluorescence emission at λfl=517±5nm when photoexcited at λex=493 nm (due to FITC) and at λfl=600±5nm when photoexcited at λex=585±5nm nm (due to AR).
What renders this TdB Labs product unique is that the fluorescence (as well as the absorbance) of one of the fluorophores (FITC) is highly dependent on pH while the corresponding ones of the other fluorophore (AR) remains nearly unchanged between pH 2 and 10 (see Fig 2). This allows for accurate determination of pH in living cells or tissues and thereby renders this product an invaluable tool for intravital microscopy.
Storage and stability
FARD20 should be stored in a well-sealed container in the dark, at room temperature.
FARD20 is delivered as a light brown to dark purple powder or crystals readily soluble in water and DMSO. However, it is incompatible with strong oxidizing agents.
FARD20 is suitable for applications requiring accurate detection and monitoring of pH in living cells or tissues, where it can operate at a wide range of pH spanning from 3.5 to 8.0.
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