Hyaluronic acid (HA) can be obtained from various sources, for example rooster combs or Streptococcus equi. We can supply derivatives of either of these qualities labelled with fluorescein (F-HA). All the batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. TdB Labs produces F-HA at a molecular weight of approximately 1.5 MDa that is supplied as a yellow powder.
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Hyaluronic acid is a polysaccharide that is composed of alternating β(1-3) glucuronide and β(1-4) glucosaminide units derived from Streptococcus equi. The hyaluronic acid is labelled with 5-amino-fluorescein. The degree of substitution lies between 0.001 and 0.008. The labelling procedure does not lead to depolymerization of the HA.
F-HA has an excitation maximum of 490 – 495 nm and an emission maximum of 520 ± 5 nm.
Storage and stability
F-HA is stable for more than 6 years when stored dry in well-sealed containers.
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Tavazzi, S. et al. Wear effects on microscopic morphology and hyaluronan uptake in siloxane-hydrogel contact lenses. Journal of Biomedical Materials Research Part B: Applied Biomaterials103, 1092–1098 (2015).
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