Fluorescein Hyaluronic Acid

Fluorescein Hyaluronic Acid

Hyaluronic acid (HA) can be obtained from various sources, for example rooster combs or Streptococcus equi. We can supply derivatives of either of these qualities labelled with fluorescein (F-HA). All the batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. TdB Labs produces F-HA at a molecular weight of approximately 1.5 MDa that is supplied as a yellow powder.

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Hyaluronic acid is a polysaccharide that is composed of alternating β(1-3) glucuronide and β(1-4) glucosaminide units derived from Streptococcus equi. The hyaluronic acid is labelled with 5-amino-fluorescein. The degree of substitution lies between 0.001 and 0.008. The labelling procedure does not lead to depolymerization of the HA.

Spectral data
F-HA has an excitation maximum of 490 – 495 nm and an emission maximum of 520 ± 5 nm.

Storage and stability
F-HA is stable for more than 6 years when stored dry in well-sealed containers.

F-HA dissolves readily in water.

F-HA has been used to study the hyaluronan uptake in siloxane-hydrogel contact lenses. Read more about applications here.


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  1. Tavazzi, S. et al. Wear effects on microscopic morphology and hyaluronan uptake in siloxane-hydrogel contact lenses. Journal of Biomedical Materials Research Part B: Applied Biomaterials 103, 1092–1098 (2015).

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