Hyaluronic acid is a polysaccharide composed of alternating β(1-3) glucuronide and β(1-4) glucosaminide units derived from Streptococcus equi. The hyaluronic acid is labelled with 5-amino-fluorescein. The degree of substitution lies between 0.001 and 0.008. The labelling procedure does not lead to depolymerisation of the HA.
F-HA has an excitation maximum at 495 nm and an emission maximum at 524 nm.
Storage and stability
F-HA is stable for more than 6 years when stored dry in well-sealed containers.
F-HA dissolves readily in water.
F-HA have been used to e.g. study the hyaluronan uptake in siloxane-hydrogel contact lenses. Read more about applications here.