Polysucrose (renamed from Ficoll™) is a polymer that displays excellent biocompatibility. Polysucrose is more sensitive to acid than dextran so that care must be taken when working at acidic pH. All the batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. TdB Labs produces FITC-polysucrose from 20 kDa to 400 kDa and these are supplied as a yellow powder.
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Polysucrose is a polymer that is synthesized by cross-linking sucrose with epichlorohydrin. FITC-polysucrose is prepared by reacting polysucrose with FITC under similar conditions to those used for FITC-dextrans. Polysucrose has a more spherical structure and is less flexible compared to dextran.
FITC-polysucrose has an excitation maximum of 490 – 495 nm and an emission maximum of 520 ± 5 nm at pH 9.
Storage and stability
FITC-polysucrose is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature.
FITC-polysucrose dissolves readily in water.
FITC-polysucrose is mainly used for studying permeability and microcirculation. Due to its spherical structure, it is often used for studies of glomerular filtration. Read more about applications here.
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