The degree of FITC substitution lies between 0.001 and 0.01. The hydroxyethyl content is on average 0.5 hydroxyethyl groups per glucose unit.
FHES (FITC-HES) has an adsorption maximum of 493 nm and an emission maximum of 520 nm.
Storage and stability
FHES (FITC-HES) is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature. FHES is also stable in aqueous solution, however, it is not recommended to make a stock solution of FHES in aqueous media for long term storage. Fresh solutions will minimise the degradation risk for the product. Store protected from light.
FHES (FITC-HES) is readily soluble in water and DMSO. Fastest dissolution is obtained by adding the dry FHES portion-wise to a stirred quantity of water. Concentrations up to 1 g per 10 mL water are possible without issues but the solution might be viscous. If solubility issues still occur, try gentle heating up to 50C and stirring with a magnetic stirring bar or
FHES (FITC-HES) is used in studies to evaluate the function and behavior of either HES or a biological system (e.g. the circulatory system, kidneys etc), or to measure blood volume. Read more about applications here.