Hydroxyethyl starch (HES) is a non-ionic starch derivative that can be of varying molecular weight. HES is also known as HAES, Tetrastarch (VoluvenTM), pentastarch (PentastanTM) and Hetastarch (HespanTM). HES may be used to treat hypovolemia or sudden blood loss. Starch is a naturally abundant (1-4 β)-Glucose polymer that is insoluble in water. However, when functionalised with hydroxyethyl groups, the starch chain becomes water-soluble and has become a useful tool in life sciences.
FITC-HES (Fluorescein isothiocyanate hydroxyethyl starch) (FHES) is manufactured by reacting FITC with HES. The product is carefully purified from reagents, solvents and by-products. It is supplied as an orange to dark orange powder. The mean average molecular weight is approx. 165 kDa.
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The degree of FITC substitution lies between 0.001 and 0.01. The hydroxyethyl content is on average 0.5 hydroxyethyl groups per glucose unit.
FITC-HES has an adsorption maximum of 493 nm and an emission maximum of 520 nm.
Storage and stability
FITC-DEAE-polysucrose is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature.
FITC-Hes is readily soluble in water and DMSO. Concentrations up to 1 g per 10 mL water are possible without issues but the solution might be viscous.
Fluorescein-HES (FHES) is used in studies to evaluate the function and behavior of either HES or a biological system (e.g. the circulatory system, kidneys etc), or to measure blood volume. Read more about applications here.
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