TRITC-dextran

TRITC -dextran

TRITC dextrans are prepared from special dextran fractions by coupling to tetramethylrhodamine B isothiocyanate (mixed isomers). The fluorescence intensity of TRITC-dextrans varies much less than with FITC-dextrans.

All batches are checked for molecular weight, degree of substitution, loss on drying and free TRITC. TdB Labs produce TRITC-dextrans from 4 kDa to 2000 kDa. TRITC-dextrans are supplied as a pink powder.

Can’t find what you are looking for? We can always produce a customised product for you. Read more here.

Structure
Dextran is a branched polysaccharide elaborated by the bacteria Leuconostoc mesenteroides. Dextran is essentially a linear glucose chain linked by α-D-(1 – 6) linkages. The degree of branching is about 5 %. The content of TRITC substituents ranges from 0.001 to 0.008 per glucose unit. At these low degrees of substitution, the charge contribution from the tertiary amino-groups on the rhodamine moiety is minimal.

Spectral data
TRITC-dextran has an excitation maximum at 550 nm and an emission maximum at 571 nm at pH 9.

Storage and stability
TRITC-dextran is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature.

Solubility
TRITC-dextran dissolves readily in water.

Application
TRITC-dextran is mainly used for studying permeability and microcirculation. It can also be used as a molecular size marker, for studies of drug delivery and more. Read more about applications here.

References

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All publications

  1. Ramirez, L.M.F.,  Gobin, E., Aid-Launais, R., Journe, C., Moraes, F.C., Picton, L., Cerf, D.L., Letourneur, D., Chauvierre, C., Chaubet, F.
    Gd(DOTA)-grafted submicronic polysaccharide-based particles functionalized with fucoidan as potential MR contrast agent able to target human activated platelets. Carbohydrate Polymers (2020) doi: https://doi.org/10.1016/j.carbpol.2020.116457.
  2. Gallego, L. D. et al. Phase separation directs ubiquitination of gene-body nucleosomes. Nature 1–6 (2020) doi:10.1038/s41586-020-2097-z.
  3. Sankaran, J. et al. Single microcolony diffusion analysis in Pseudomonas aeruginosa biofilms. npj Biofilms Microbiomes 5, 1–10 (2019).
  4. Fawke, S. et al. Glycerol‐3‐phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of the tomato and Nicotiana benthamiana leaf epidermis. New Phytol nph.15846 (2019) doi:10.1111/nph.15846.
  5. Brooks, J. et al. Development of Kinetic Modeling to Assess Multi-functional Vascular Response to Low Dose Radiation in Leukemia. bioRxiv 633644 (2019) doi:10.1101/633644.
  6. Palygin, O. et al. Nitric oxide production by glomerular podocytes. Nitric Oxide 72, 24–31 (2018).
  7. Wang, L., Kamocka, M. M., Zollman, A. & Carlesso, N. Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches. JoVE (Journal of Visualized Experiments) e54253 (2017) doi:10.3791/54253.
  8. Endres, B. T. et al. Intravital imaging of the kidney in a rat model of salt-sensitive hypertension. American Journal of Physiology-Renal Physiology 313, F163–F173 (2017).
  9. Collett, J. A. et al. Hydrodynamic Isotonic Fluid Delivery Ameliorates Moderate-to-Severe Ischemia-Reperfusion Injury in Rat Kidneys. JASN 28, 2081–2092 (2017).
  10. Bulant, C. A., Blanco, P. J., Müller, L. O., Scharfstein, J. & Svensjö, E. Computer-aided quantification of microvascular networks: Application to alterations due to pathological angiogenesis in the hamster. Microvascular Research 112, 53–64 (2017).
  11. Jiang, M.-Y. et al. The microfluidic synthesis of composite hollow microfibers for K+-responsive controlled release based on a host–guest system. J. Mater. Chem. B 4, 3925–3935 (2016).
  12. Choi, M., Lee, W. M. & Yun, S. H. Intravital Microscopic Interrogation of Peripheral Taste Sensation. Scientific Reports 5, 8661 (2015).
  13. Laudien, J. et al. Perfluorodecalin-soluble fluorescent dyes for the monitoring of circulating nanocapsules with intravital fluorescence microscopy. Journal of Microencapsulation 31, 738–745 (2014).
  14. Corridon, P. R. et al. A method to facilitate and monitor expression of exogenous genes in the rat kidney using plasmid and viral vectors. American Journal of Physiology-Renal Physiology 304, F1217–F1229 (2013).

How to order

Visit our webshop to see the molecular weights and pack sizes available. Please send an e-mail to order@tdblabs.se if you would like to receive a quote, place a bulk order or if you wish to place your order manually.

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