FITC -CM-dextran

FITC-CM-dextrans are standard FITC-dextran carrying carboxymethyl-substituents. FITC-CM-dextrans have been developed for special studies on membranes and cells. The CM (carboxymethyl) group gives the product anionic (negative) charge. All batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. TdB labs produce FITC-CM-dextrans from 4 kDa to 150 kDa. FITC-CM-dextran is supplied as a yellow powder.

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Structure
FITC-CM-dextrans are manufactured by reacting selected dextran fractions with an activated carboxymethyl derivative in alkali whereby O-carboxymethyl groups are introduced along the dextran chain. The carboxyl content is approximately 5% which is equivalent to about one CM group for every five glucose units. Thereafter, fluorescein (FITC) groups are introduced by reaction with fluorescein isothiocyanate. The degree of substitution of FITC lies between 0.003 – 0.008.

Storage and stability
FITC-CM-dextran is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature.

Solubility
FITC-CM-dextran dissolves readily in water.

Application
FITC-CM-dextran are mostly used for studies of permeability and microcirculation. The carboxyl groups will impart an overall negative charge to the molecule, which may be valuable in gaining information on the permeability characteristics of cell membranes and tissues. The free carboxyl group is also useful for coupling other substances to the dextran chain. Read more about applications here.

References

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How to order

Visit our webshop to see the molecular weights and pack sizes available. Please send an e-mail to order@tdblabs.se if you would like to receive a quote, place a bulk order or if you wish to place your order manually.