FITC-CM-dextrans are standard FITC-dextrans carrying carboxymethyl-substituents. FITC-CM-dextrans have been developed for special studies on membranes and cells. The CM (carboxymethyl) group gives the product anionic (negative) charge. All batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. TdB labs produce FITC-CM-dextrans from 4 kDa to 150 kDa. FITC-CM-dextran is supplied as a yellow powder.
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FITC-CM-dextrans are manufactured by reacting selected dextran fractions with an activated carboxymethyl derivative in alkali whereby O-carboxymethyl groups are introduced along the dextran chain. The carboxyl content is approximately 5% which is equivalent to about one CM group for every five glucose units. Thereafter, fluorescein (FITC) groups are introduced by reaction with fluorescein isothiocyanate. The degree of substitution of FITC lies between 0.003 – 0.008.
FITC-CM-dextran has an excitation maximum of 490 – 495 nm and an emission maximum of 520 ± 5 nm.
Storage and stability
FITC-CM-dextran is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature.
FITC-CM-dextran dissolves readily in water.
FITC-CM-dextrans are mostly used for studies of permeability and microcirculation. The carboxyl groups will impart an overall negative charge to the molecule, which may be valuable in gaining information on the permeability characteristics of cell membranes and tissues. The free carboxyl group is also useful for coupling other substances to the dextran chain. Read more about applications here.
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Li, B. et al. Functionalized polymer microbubbles as new molecular ultrasound contrast agent to target P-selectin in thrombus. Biomaterials194, 139–150 (2019).
Chen, Y., Zhang, M. & Ren, F. A Role of Exopolysaccharide Produced by Streptococcus thermophilus in the Intestinal Inflammation and Mucosal Barrier in Caco-2 Monolayer and Dextran Sulphate Sodium-Induced Experimental Murine Colitis. Molecules24, 513 (2019).
Kojima, T. & Takayama, S. Patchy Surfaces Stabilize Dextran–Polyethylene Glycol Aqueous Two-Phase System Liquid Patterns. Langmuir29, 5508–5514 (2013).
Srikantha, N. et al. Influence of molecular shape, conformability, net surface charge, and tissue interaction on transscleral macromolecular diffusion. Exp. Eye Res.102, 85–92 (2012).
Asgeirsson, D., Venturoli, D., Rippe, B. & Rippe, C. Increased glomerular permeability to negatively charged Ficoll relative to neutral Ficoll in rats. Am. J. Physiol. Renal Physiol.291, F1083-1089 (2006).
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