FITC-inulin is synthesized by reacting fluoresceinyl isothiocyanate (FITC) with inulin from dahlia tubers. The product is a yellow powder that is readily soluble in water. All the batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. TdB Labs produces FITC-inulin at a molecular weight of 4.5 kDa.
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An inulin fraction obtained from dahlia tubers is labelled with fluorescein. The fluorescein moiety is attached by a stable thiocarbamoyl linkage and the labelling procedure does not lead to any depolymerization of inulin. FITC-inulin possesses from 0.001-0.008 mol FITC per fructose unit. At these low levels of substitution, the effect of the charges is minimal.
FITC-inulin has an excitation maximum of 490 – 495 nm and an emission maximum of 520 ± 5 nm.
Storage and stability
FITC-inulin is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature.
FITC-inulin dissolves readily in water.
Inulin has long been used for monitoring renal clearance. FITC-inulin offers a simple and sensitive alternative to the cumbersome chemical assays of inulin and permits real time analyses. Read more about applications here.
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