FITC-inulin

FITC -inulin

FITC-inulin is synthesised by reacting fluoresceinyl isothiocyanate (FITC) with inulin from dahlia tubers. The product is a yellow powder which is readily soluble in water. All batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. TdB Labs produce FITC-inulin with a molecular weight of 4 kDa. FITC-inulin is supplied as a yellow powder.

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Structure
An inulin fraction obtained from dahlia tubers is labelled with fluorescein. The fluorescein moiety is attached by a stable thiocarbamoyl linkage and the labelling procedure does not lead to any depolymerization of inulin. FITC-inulin possesses from 0.001-0.008 mol FITC per fructose unit. At these low levels of substitution, the effect of the charges is minimal.

Spectral data
The excitation maximum for FITC-inulin is 490 nm and the emission maximum is 520 nm. Fluorescence is dependent on pH.

Storage and stability
FITC-inulin is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature

Solubility
FITC-inulin dissolves readily in water.

Application
Inulin has long been used for monitoring renal clearance. FITC-inulin offers a simple and sensitive alternative to the cumbersome chemical assays of inulin and permits real time analyses. Read more about applications here.

References

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